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INTRODUCTION TO NGS SEQUENCING METHODS AND APPLICATIONS IN GENOMICS, METAGENOMICS AND TRANSCRIPTOMICS

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Objectives :

To understand the principles of Next Generation Sequencing (NGS) technology and explore its applications in OMICs data analyses.

Level of trainees :

Open to all (biologists, biostatisticians, project managers etc.).

Prerequisites :

Basic knowledge of biology and statistics is helpful.

Duration and location :

• Duration : 7 hours over 1 day
• Location : Soladis training center in Paris or Lyon (to consult the calendar, please click on “Sign up”) or directly at your site (minimum of 4 people)

Training fee:

• At Soladis training center : 700€ tax-free per trainee
• Other : Contact us for an offer adapted to your company.

Soladis invites you to participate in this training course on NGS sequencing methods and applications in genomics, metagenomics and transcriptomics. This training addresses all biologically oriented people who wish to understand and deepen their knowledge on the subject in order to launch and carry out NGS data mining projects.

Training Schedule

INTRODUCTION

• History of Sequencing technologies: From Sanger to Single Cell
• Evolution of big-data applications

Next Generation SEQUENCING

• Understand the concepts of different NGS sequencing methods
• Characteristics of a sequencing method: sequencing time, length of reads, depth, and choice of libraries etc.

SEQUENCING DATA PROCESSING

• Understanding the raw outputs of sequencers
• Ensure data quality control
• Genome data analysis: de novo assembly vs mapping
• Overview of the main databases

EXAMPLES OF NGS APPLICATIONS

• Bacterial epidemiology and comparative genomics
• Differential analysis in oncology and signature research
• Targeted metagenomics in cosmetology
• Functional metagenomics of the intestinal microbiota

APPLICATION TO CLASSICAL CASES

• Sharing and discussion through hands-on application examples

Do not hesitate to contact us for more information about this training, which can be organized in our training center (dates provided on our website) or directly in your premises, according to your preferences.